Characterization of the ceramide moieties of sphingoglycolipids from mouse brain by ESI-MS/MS: identification of ceramides containing sphingadienine.
نویسندگان
چکیده
Sphingoglycolipids (SGLs) are cell membrane constituents. As the ceramide structure influences the biological properties of the SGL, we characterized by electrospray ionization tandem mass spectrometry the molecular species of ceramides present in SGL of mouse brain. We report here for the first time the presence in mammalian brain of sphingadienine (d18:2). Sphingenine (d18:1) is present in all SGL species, in contrast to eicosasphingenine (d20:1), which is a constituent of only gangliosides. Sphingadienine is present in galactosylceramide and sulfatides. Free ceramides contain the three types of bases. Thus, there could be two separate pools of free ceramides (d18:1, d18:2 and d20:1, d18:1) as precursors of complex SGL.
منابع مشابه
Characterization of the ceramide moieties of sphingoglycolipids from mouse brain by electrospray-tandem mass spectrometry: identification of ceramides containing sphingadienine
Sphingoglycolipids (SGL) are cell membrane constituents. As the ceramide structure influences the biological properties of the SGL, we characterized by electrospray tandem mass spectrometry the molecular species of ceramides present in SGL of mouse brain. We report here for the first time the presence in mammalian brain of sphingadienine (d18:2). Sphingenine (d18:1) is present in all SGL specie...
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عنوان ژورنال:
- Journal of lipid research
دوره 45 2 شماره
صفحات -
تاریخ انتشار 2004